ABSTRACT
OBJECTIVE: To observe the effects of recipe for invigorating kidney on expression of estrogen receptors in ovariectomized rats. METHODS: The rats, which were given high-cholesterol diet after ovariectomy, were chosen as experimental subjects. After fed kidney-invigorating recipe for 16 weeks, the rats were killed and the levels of serum lipid and estradiol of these rats were examined. The expressions of estrogen receptor alpha and beta mRNAs in aorta, pituitary gland and hypothalamus, and the affinity of estrogen receptor-ligand were also detected. RESULTS: The kidney-invigorating recipe could increase the expression levels of estrogen receptor alpha and beta mRNAs in different parts such as aorta, pituitary gland and hypothalamus, and enhance the affinity of estrogen receptor-ligands. CONCLUSION: The recipe for invigorating kidney may meliorate atherosclerosis in ovariectomized rats by regulating the estrogen receptors.
ABSTRACT
OBJECTIVE: To establish an animal model imitating post-menopausal atherosclerosis in rabbit and observe the effect of the herbal formula of tonifying-kidney on this animal model. METHODS: The New Zealand rabbits were treated with ovariectomy, high cholesterol diet plus immune injury, and both of ovariectomy and high cholesterol diet plus immune injury respectively. The blood lipid level and the intima and media of the aorta were observed. RESULTS: The rabbits treated with ovariectomy and high cholesterol diet plus immune injury changed markedly and had low level of estrogen, high levels of CHOL, TG and dysfunction of the arterial endothelium cells. however, the chinese herbal formula of tonifying-kidney could relieve these changes. CONCLUSION: The New Zealand rabbits treated with both of ovariectomy and high cholesterol diet plus immune injury could result in the changes imitating post-menopausal atherosclerosis, and it could be improved by the treatment of herbal formula of tonifying-kidney, so we think that the changes of the animal model are related to the deficiency of the kidney in traditional Chinese medicine.
ABSTRACT
Objective:The effect of “BU SHEN Decoction” on serum lipid, nitric oxide (NO), endothelin (ET), and micro structure of the endothelial cell was observed in experimental post menopause atherosclerosis rabbits.Methods: The post menopause atherosclerosis rabbit model was established by feeding high cholesterol diet after the rabbits had been given the castration operation and injected with bovine serum albumin. After “BU SHEN Decoction” had been given for 12 weeks, the blood sample was collected. Results: “BU SHEN Decoction” can decrease the serum cholesterol (CHO), triglyceride (TG)( P